native HisTag elution alternative

[Richard P. Grant]

In article <v04011701b61f37a81c73@[144.92.64.179]>, 
mlsulliv at facstaff.wisc.edu ("Michael L. Sullivan") wrote:

> Several vendors suggest 100 mM EDTA pH 8.0 as an EDTA elution condition. 

If memory serves, then surely low mM DTT will bring it off.

Might knacker the column completely, of course :)


R

-- 
Richard P. Grant                                     MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk   http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
    "If it's not on fire, it's a software problem" - Matt Burch