native HisTag elution alternative

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Fri Oct 27 09:59:13 EST 2000


>In article <v04011701b61f37a81c73@[144.92.64.179]>,
>mlsulliv at facstaff.wisc.edu ("Michael L. Sullivan") wrote:
>
>> Several vendors suggest 100 mM EDTA pH 8.0 as an EDTA elution condition.
>
>If memory serves, then surely low mM DTT will bring it off.
>
>Might knacker the column completely, of course :)

Just happen to have the book from Novagen's product on my desk.  They
indicate that DTT can be used up to 1 mM, but they don't really recommend
it routinely in buffer.  Seems then that higher concentrations could be
used for elution then, doesn't it.
>


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