Immunoprecipitation with non-immune serum??

Neal Robert Melvin nrmelvin at ucalgary.ca
Sun Oct 29 02:55:27 EST 2000


Hi, I'm starting to do IPs with a rabbit polyclonal antisera. I plan to
use a non-immune rabbit antisera as a control, and in searching the
literature, have noticed something strange... I've seen people that have
used non-immune serum, and have a lane that is completely blank after
detection with an anti-rabbit antibody... my question is: shouldn't the
heavy/light chains of the non-immune serum be detected on a western blot
when using an anti-rabbit secondary?? Doesn't seeing absolutely nothing in
the non-immune serum lane indicate that the rabbit antibodies didn't bind
to the protein A in the first place??

"If there are many universes, there will be one where there is a set of
numbers suitable to life... we are in that one"

Martin Rees, Astronomer Royal 

Neal Melvin
University of Calgary - Faculty of Medicine
Department of Neuroscience - Neuroscience Research Group (NRG)
Health Sciences Centre
3330 Hospital Drive, NW
Calgary, Alberta, Canada
T2N 4N1

e-mail: nrmelvin at ucalgary.ca
Phone: (403) 220-4490 (lab)
       (403) 220-7035 (office)
       (403) 283-8731 (fax)
	










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