growth of MCF10A mammary epithelial cells

Martin Offterdinger martin.offterdinger at akh-wien.ac.at
Mon Oct 30 02:58:03 EST 2000


"Alexander Dobrovic" <alexander.dobrovic at adelaide.edu.au> schrieb im
Newsbeitrag news:l03130302b61555128c1f@[129.127.90.187]...
> We wish to grow some MCF10A mammary epithelial cells. These seem to
require
> a fairly complex medium according to ATCC. Has anyone used a simpler
medium
> with success.
>
> Regards
>
> Alex
>
>
> alexander.dobrovic at adelaide.edu.au
>
> 61-(0)8-8222 7676
>
>
>

I used the complex medium, (both serum-free and serum-containing) and found
out the following:

1)You can omit the choleratoxin; cells still grown although a little slower.
2) They also grown in high glucose DMEM with 10%FCS, PenStrep, supplemented
with 5ug/ml Hydrocortisone and 10ug/ml Insulin

Hope this is of some help to you.
martin








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