search for a device allowing both dialysis and smoothconcentration

nospam at our.site nospam at our.site
Mon Oct 30 10:29:20 EST 2000


This message has been posted by:  612 right <i.hinners at REMOVE-THIS-TO-SENDicrf.icnet.uk>

We used to get the membranes from Schleicher & Schuell, (2-3 years ago)
dont know if the do the apparatus as well?

Louis RENAULT wrote:

> Dear Colleagues,
> We are looking for a device that allows
> both protein dialysis and smooth concentration by atmospheric pressure.
>
> Here is the kind of setup we refer to:
> Dialysis is made inside dialysis membranes
> in a bottle/reservoir (500-1000 ml) which can be closed
> hermetically. One extremity of the membrane is not
> in the solution but is opened to the air.
> Concentration starts with this set-up
> by introducing vacuum inside the closed bottle, then the atmospheric
> pressure pushes the sample inside the dialysis membrane
> and concentrates it VERY SMOOTHLY.
>
> This double function of the device is especially appropriate
> -to test a large variety of buffers for solubilizing proteins or protein
>    complexes to high concentration (10-20 mg/ml at least) when these samples are
>    poorly-soluble and fragile in solution
> -to avoid protein denaturation or that most of the protein (50 to 70%)
>    stick to the membrane upon concentration by centrifugation
>    (it happens for us with centricon, microcon, ... systems)
> -to obtain the highest possible concentration of the sample
>    in a buffer containing a minimum content of salt (less than 50 mM)
>
> Such devices were previously sold
> at MERCK or Fischer companies (Micro-ProDICon device)
> or Sartorius (Kollodiumhülsen-Glashalter)
> but are not available any more.
>
> Does anyone know where we can buy such device ?
>
> Any help will be greatly appreciated
> yours sincerely,
> louis
> --
> *******************************************************************
> **   Louis RENAULT                                               **
> **   Laboratoire d'Enzymologie et Biochimie Structurales (LEBS)  **
> **   Bat. 34, CNRS,                                              **
> **   1, avenue de la Terrasse                                    **
> **   91 198 Gif-sur-Yvette cedex, FRANCE                         **
> **                                                               **
> **   Phone: 33-1 69 82 42 47 (inside France: 01 69 82 42 47)     **
> **   Fax: 33-1 69 82 31 29                                       **
> **   email: louis.renault at lebs.cnrs-gif.fr                       **
> *******************************************************************
> .
>
> ---

--
Ina Hinners
ICRF
Secretory Pathways Laboratory
44 Lincolns Inn Fields
WC2A 3PX London
UK
email: I.Hinners at icrf.icnet.uk







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