alcohol in antibiotic solutions?
ekhatipo at NOSPAMmidway.uchicago.edu
Mon Oct 30 22:49:20 EST 2000
Let's just calculate. Say, you have 1 ml of 20mg/ml soln of Cm in EtOH. You
add 25ul of that soln to 10ml of LB to make up to 50ug/ml Cm. Your finally
EtOH concentration in the medium is ~50mM then. How miniscule it is? 2g/l of
glucose for example is just about 10mM. The only good thing about it is that
to my understanding E.coli cannot utilize EtOH when grown aerobically.
Still, the presence of 50mM alcohol should have a certain effect on, say,
the length of the lag phase when you re-inoculate your o/n culture into
fresh LB-antibiotic medium.
""Dr. Hiranya S. Roychowdhury"" <hroychow at nmsu.edu> wrote in message
news:188.8.131.52.20001030200947.007c0490 at cnmailsvr.nmsu.edu...
> At 11:36 PM 10/30/00 -0000, Rodney Pettway wrote:
> > Does it all volatilize off or what?
> Yes; and even if it didn't, the amount would be too miniscule to affect
> Dr. Hiranya Sankar Roychowdhury
> College Asst. Prof.
> Molecular Biology,
> Dept. of Chemistry & Biochemistry
> Box 30001 - 3MLS
> New Mexico State University
> Las Cruces, NM 88003
> Lab: (505) 646 4722
> Office: (505) 646 8256
> hroychow at nmsu.edu
More information about the Methods