problems with GST-fusion protein

Silke Meister silke.meister at molmed.uni-erlangen.de
Fri Sep 1 08:30:29 EST 2000


I cloned my protein in the pGEX-vector and wanted to purify this
GST-fusion protein, but I wasn't succesful!
The protein didn't bind to the sepharose-beads. In my opinion the
protein prevent the binding from GST to GSH, because the empty
GST-vector without the protein can bind!
I hope you can help me to solve my problem!


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