direct gfp observation

bturner at caregroup.harvard.edu bturner at caregroup.harvard.edu
Wed Sep 6 10:07:47 EST 2000


>From bsturner at mbcrr.harvard.edu Thu Aug 31 19:56 EDT 2000 
Date: Thu, 31 Aug 2000 19:56:17 -0400 (EDT) 
From: Bradley Turner <bsturner at mbcrr.harvard.edu> 
X-Sender: bsturner at mbcrr 
To: MOUNIR IZALLALEN <aah313 at agora.ulaval.ca> 
cc: methods at hgmp.mrc.ac.uk 
Subject: Re: direct gfp observation 
MIME-Version: 1.0 
X-Status: 



Hello Mounir, 



Clare Chemical <http://www.clarechemical.com/> produces 
an item called the "Dark Reader" that emits *visible* blue 
light (~450nm) and excites EtBr, Sybr dyes, and GFP at 
this secondary excitation wavelength avoiding UV hazards. 
Their web site has lots of information and GFP and DNA gel 
pictures. 

Also, AlphaInnotech <http://www.alphainnotech.com/> supplies 
a "UV to Chromalight blue" conversion screen that will 
convert any standard UV transilluminator into one that 
emits around 460nm (visible blue) and will excite the above 
fluors. 

Yet another company that makes a similar product albeit 
a little more sophisticated (i.e. more expensive) is 
Light Tools Research <http://www.lightools.com/> 
Their web site also has lots of information and GFP 
pictures. 

With all of these I think that you will also need an 
orange filter that they will supply.  Supposedly it is 
easier to see GFP containing bacteria on plates with 
blue light because there is no autofluorescence of the 
agar/media that is seen with UV excitation, and of course 
there is much less DNA damage. 

Hope this helps, 
Brad Turner [no affiliations] 



**************************************************************** 
                    Bradley Turner 
                Beth Israel Deaconess 
                    Medical Center 

Harvard Medical School          617-667-1215 phone 
Division of Gastroenterology    617-667-2767 fax 
Room Dana 605                   bsturner at biosun.harvard.edu 
330 Brookline Avenue            bturner at caregroup.harvard.edu 
Boston, MA 02215                bsturner at mbcrr.harvard.edu 
**************************************************************** 





On Thu, 31 Aug 2000, MOUNIR IZALLALEN wrote: 

> Hi, 
> 
> Do you know of any mean to observe gfp-producing bacterial colonies on 
> agar plate without using UV light. 
> 
> Thank you, 
> 
> Mounir 
> 
> 
> 



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