Problems of electroporation.

Helen Mok helenmok at yahoo.com
Wed Sep 6 10:20:33 EST 2000


Does anyone have experience of successful
transformation of control plasmid into E. coli with
electroporator, but nothing resulted with ligated DNA
(after ethanol precipitation)??

The E. coli I use is DH12S (Life Tech) and Gene Pulser
from BioRad (0.2cm cuvette, 1.8kV, 25uF, 200 ohm).

All replies would be appreciated! Thanks a lot!!

Helen Mok
City University of Hong Kong

helenmok at yahoo.com

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