TOP10- good strain for DNA sequencing?

Student mucineer at
Thu Sep 7 04:35:26 EST 2000

Has anyone had any problems with sequencing plasmid vectors cloned in E coli
TOP10 (Invitrogen)? I have found that it gives me very low signals from time
to time and sometimes the signals tail off prematurely. Has this got
anything to do with the carbohydrates it produces? Is there anyway to get
round this apart from using another strain? Which ones are good in this
repsect. Many thanks.

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