TOP10- good strain for DNA sequencing?

Bas Jansen basjhj at nospam.nl
Thu Sep 7 08:10:52 EST 2000


Neil Saunders heeft geschreven in bericht
<8p7ven$9r$1 at mirv.comms.unsw.edu.au>...


[...]

>Strains are one of those funny things where everyone has a personal
>experience.  I'd say a good quality kit will overcome strain problems most
>times (eg Promega Wizard).  Have a read of the Promega or Qiagen
literature,
>they normally talk about preferred strains for plasmid sequencing.
>Personally I've found TOP10 OK but it wouldn't be top of my list.  XL1-Blue
>is often touted as a star performer in this respect.


I remember a Perkin Elmer dude saying that it is best to use DH10B or the
good ol' DH5alpha. Definitely not the JM or HB series, in any case. We've
also had some trouble with TOP10 cells.

All the best,
Bas









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