EtOH ppt of DNA, what's wrong with this strategy?
SPAMFREEobrien at pharm.med.upenn.edu
Thu Sep 7 00:46:44 EST 2000
There's got to be a reason for adding NaOAc to the aqueous DNA solution
BEFORE adding the EtOH. Is this an absolute necessity?
I'm doing >1000X precipitations/day of PCR products in 96 well plates, and
am trying to save time, effort, and pipette tips in any way possible. Will
the ppt still work if I make a mix of the 95% EtOH and NaOAc then add it all
Any help greatly appreciated. Please email as well as posting.
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