EtOH ppt of DNA, what's wrong with this strategy?

Chris LaRosa clarosa at biocomp.unl.edu
Thu Sep 7 13:01:06 EST 2000



Peter O'Brien wrote:
> 
> There's got to be a reason for adding  NaOAc to the aqueous DNA solution
> BEFORE adding the EtOH.  Is this an absolute necessity?
> 
> I'm doing >1000X precipitations/day of PCR products in 96 well plates, and
> am trying to save time, effort, and pipette tips in any way possible.  Will
> the ppt still work if I make a mix of the 95% EtOH and NaOAc then add it all
> at once?
> 
>I have been mixing the NaOAC and ethanol together for ppt of pcr products.... no problems have been observed when it is done for pcr products and for sequencing products...






More information about the Methods mailing list