Summary & References: freeze-thaw sucrose gradients
klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Thu Sep 7 20:07:31 EST 2000
bsturner at mbcrr.harvard.edu (Bradley Turner) wrote:
>Thank you to everyone for your help in tracking down information
>and sharing protocols about a very interesting technique for
>(mass)producing sucrose gradients. Below I've listed the various
>methods that were sent to me along with some references from
>Medline that I found on the topic. I hope others will also
>find this helpful.
You also requested an explanation why they form. Have you got one?
It's really simple: 1) ice floats, 2) freezing point of most solutions is
below freezing point of water; the more solute, the less freezing point.
The two combined make you a nice gradient if you freeze slowly
More information about the Methods