kinase pb in aflp radiolabelling

LAURENT MARCHÉ marche at lerheu.rennes.inra.fr
Fri Sep 8 07:50:10 EST 2000


Hello,

We have a problem with radiolabelling with gamma P33 for AFLP.

We have worked with both Boehringer and Gibco T4 kinase phosphatase free.

  Is there any difference between them ?

  Our labelling first worked with Boehringer, but not now.

Is the T4 kinase Gibco from AFLP kit has only to be used with specific 
gibco kit products (primers for example)?

Thanks for answers

LAURENT MARCHÉ marche at rennes.inra.fr

INRA/ENSAR UMR Bio3P

Unité Mixte de Recherche en Biologie des Organismes
et des Populations appliquée à la Protection des Plantes

Domaine de la Motte

35653 LE RHEU FRANCE

tel : 0033(0)223485159
Fax : 0033(0)223485150


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