GFP in periplasm

MIke News benedik at
Sun Sep 10 14:38:03 EST 2000

   Has anyone expressed GFP in the periplasm of gram negative bacteria such
as E. coli? I had planned some experiments to do so, but then heard that GFP
fails to work in the periplasm. Can anyone substantiate or disprove that

Many thanks


Michael Benedik
Dept of Biology and Biochemistry
University of Houston
emailto:benedik at

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