Puzzled by in-gel kinase assay

Karthik Aghoram kaghoram at unity.ncsu.edu
Mon Sep 11 10:00:49 EST 2000


Hi nettors,

We routinely do in-gel kinase assays in our lab, but no one has an answer 
to this question:

Why does the protein in the matrix not migrate during electrophoresis to
make a discreet "band" through the gel? 

Any insights?

Thanks,
KArthik






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