DNA-binding protein

Rogier rogier666 at my-deja.com
Tue Sep 12 04:45:32 EST 2000


I'd start with UV crosslinking. Mix protein with radioactive DNA and/or
RNA (labeled plasmids, labeled PCR products,labeled in vitro
transcribed RNA, whatever you like), put in the stratalinker (2 runs at
maximum power), treat with DNase/RNase, run on SDS-PAGE, see if your
protein gets labeled this way.
Some people manage to do the trick with DIG-labeled DNA/RNA, so you
don't need to play it radioactive.
Good luck,
Rogier

--
Rogier Stuger
Dept MicFizz, Free U of A
E rogier AT biogate DOT com (spammers will be killed)
do NOT use the my-deja.com address. I never read that one


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