Problem: removal of DNA from RT-PCR using DNase

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Sep 12 06:23:52 EST 2000


In article <8pk1bq$u57$1 at nnrp1.deja.com>, teresa_mogul at my-deja.com
writes
>I measured the amount of RNA/DNA in
>my sample and according to the manifacturers notes 30 minutes at 37°C
>with 1U DNase should be ample to remove my DNA. 

Make sure you are using an Mn containing buffer and not an Mg containing
buffer for this digestion.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk






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