Problem: removal of DNA from RT-PCR using DNase

Dr. Duncan Clark Duncan at
Tue Sep 12 06:23:52 EST 2000

In article <8pk1bq$u57$1 at>, teresa_mogul at
>I measured the amount of RNA/DNA in
>my sample and according to the manifacturers notes 30 minutes at 37°C
>with 1U DNase should be ample to remove my DNA. 

Make sure you are using an Mn containing buffer and not an Mg containing
buffer for this digestion.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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