Short sequences!

T V Naga Rama Chander chander at kelvin.ncl.res.in
Tue Sep 12 12:52:49 EST 2000


hi!
is it gc rich seq?
whether or not use dmso in the sample. search for the article.
if i get it i w'd post it.

usuallu g+c rich seq are difficult to read.
we ahd the same problem earlier.

bye
rc

On 12 Sep 2000, Carlos Vergara wrote:

> Hello, you all!
>=20
> We have been experiencing problems with our ABI 377.
> Over the last weeks, we have seen a continual decline in our sequencing s=
amples quality gave short sequences that the signals decreased after 300 bp=
=2E
> We are using good DNA template extracted by phenol/chlorophorm method,
> very well PCR products purified from low melting point agarose gel with g=
elase,
> and cycle sequencing products cleaned by sephadex columns.=20
> Recently, our ABI 377 was checked and no found it technical problems.
>=20
> Does any have experience, comments or solution to the problem?
>=20
> Thanks for all kind responses in advance!
>=20
> Carlos Vergara
> vergarac at naos.si.edu
> Panam=E1, Rep=FAblica de Panam=E1
>=20
>=20
> ---
>=20
>=20

plant tissue culture division=09     !
national chemical laboratory=09     !
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india.=09=09=09=09     !
tel: 91-20-5893300 ext: 2219         !
fax: 91-20-5893438=09=09     !
e-mail: chander at kelvin.ncl.res.in    !


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