Which strand of a plasmid is replicated?
Wolfgang.Schechinger at med.uni-tuebingen.de
Wed Sep 13 03:08:17 EST 2000
I must be more precise. Of course, both strands will be replicated,
but I'd like to know wich strand is used as template for copying.
I.e. if I have a mutation in *one* strand (e.g. introduced by a
primer extension PCR with *one* primer), will it be replicated in
any case or only when it is in the upper or lower strand?
> Hi Wolfgang,
> Wolfgang Schechinger wrote:
> > Hi all,
> > Does anyone know which strand of a plasmid is replicated in E.
> > coli K12 strains (I currently use 298)?
> > All input is welcome,
> Ummmm, aren't both strands of a double stranded plasmid
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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de
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