Which strand of a plasmid is replicated?

[Wolfgang Schechinger]

Hi John, 

I must be more precise. Of course, both strands will be replicated, 
but I'd like to know wich strand is used as template for copying.
I.e. if I have a mutation in *one* strand (e.g. introduced by a 
primer extension PCR with *one* primer), will it be replicated in 
any case or only when it is in the upper or lower strand?

Wolfgang

> Hi Wolfgang,
> 
> Wolfgang Schechinger wrote:
> 
> > Hi all,
> >
> > Does anyone know which strand of a plasmid is replicated in E.
> > coli K12 strains (I currently use 298)?
> >
> > All input is welcome,
> 
> Ummmm, aren't both strands of a double stranded plasmid
> replicated?
> 
> John
> 
> 
> 
> 
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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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usual disclaimers apply 
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