Which strand of a plasmid is replicated?

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Wed Sep 13 09:20:25 EST 2000


In article <200009130807.KAA08761 at intwww.zit.med.uni-tuebingen.de>,
Wolfgang Schechinger <Wolfgang.Schechinger at med.uni-tuebingen.de> writes
>Of course, both strands will be replicated, 
>but I'd like to know wich strand is used as template for copying.
>I.e. if I have a mutation in *one* strand (e.g. introduced by a 
>primer extension PCR with *one* primer), will it be replicated in 
>any case or only when it is in the upper or lower strand?

I'd say both. You can make an in vitro mutagenesis reaction using ds
plasmid and make the mutation on either strand. You should get a 50:50
mutation:wt ratio regardless of which strand was primed initially.

Is this what you are asking?

Duncan  
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk






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