Carbonate-based blotting transfer solution recipie?

Gerhard Schwall gschwall at iuct.fhg.de
Wed Sep 13 10:46:37 EST 2000


Try this one:
10 mM NaHCO3, 3 mM Na2CO3, (+ Methanol + trace of SDS as in the Tris
Glycine buffer system), do not adjust the pH
Ref: Dunn, SD. Anal. Biochem. 157, 144 (1986)

Good luck
Gerhard

Dima Klenchin schrieb:

> Hello,
>
> A while ago I've seen a post that referenced a paper about
> the subject. The claim was it is a lot cheaper and works better
> than Tris/Glycine and on a par with CAPS-based solutions.
> We do awful lot of Westerns and although the most expensive
> part of them is probably methanol, Tris, Glycine and particularly
> CAPS are not cheap. It would be great if something as simple
> as baking soda can used.
>
> Can anyone provide a recipie, hints or a reference?
>
> - Dima

--
Dr. Gerhard Schwall
Fraunhofer Institut
Umweltchemie und Ökotoxikologie
Abt. Molekulare Biotechnologie
Auf dem Aberg 1
D-57392 Schmallenberg, Germany

e-mail: gschwall at iuct.fhg.de
Tel.: +49(0)2972 - 302242 or -302238
Fax:  +49(0)2972 - 302368







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