immunoprecipitation

Lihua He lhe at email.unc.edu
Wed Sep 13 09:32:54 EST 2000


Hello, there:
I have a question of immunoprecipitation of mitochondrial membrane
protein.
I am interested in what protein(s) are bound to this mitochondrial
membrane protein. So I don't want to disrupt the protein complex before
IP but I have to dissociate the proteins from the membrane. Is there a
way I can use IP for this kind of situation? Or is there any other
method than IP that I can use. You input is greatly appreciated.

Thanks.

Lihua He

Department of Cell Biology and Anatomy
Univ. of North Carolina at Chapel Hill
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