Carbonate-based blotting transfer solution recipie?
klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Wed Sep 13 21:38:56 EST 2000
In article <39BFA15D.A1900A2C at iuct.fhg.de>, Gerhard Schwall <gschwall at iuct.fhg.de> wrote:
>Try this one:
>10 mM NaHCO3, 3 mM Na2CO3, (+ Methanol + trace of SDS as in the Tris
>Glycine buffer system), do not adjust the pH
>Ref: Dunn, SD. Anal. Biochem. 157, 144 (1986)
Many thanks! I'll go and read the paper.
>Dima Klenchin schrieb:
>> A while ago I've seen a post that referenced a paper about
>> the subject. The claim was it is a lot cheaper and works better
>> than Tris/Glycine and on a par with CAPS-based solutions.
>> We do awful lot of Westerns and although the most expensive
>> part of them is probably methanol, Tris, Glycine and particularly
>> CAPS are not cheap. It would be great if something as simple
>> as baking soda can used.
>> Can anyone provide a recipie, hints or a reference?
>> - Dima
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