Antifoam in DNA isolation?
Dr. Peter Gegenheimer
PGegen at UKans.nolospamare.edu
Thu Sep 14 15:07:06 EST 2000
On Thu, 14 Sep 2000 10:44:34, Vince.Mulholland at sasa.gov.uk (Vince Mulholland)
wrote:
ð I want to use a mixer-mill to disrupt plant leaf material for DNA
ð isolation. The lysis solution I want to use contains SDS, so will
ð produce a lot of bubbles. If I can't minimise the formation of bubbles
ð there will be a real risk of cross-contamination of the DNA (for use in
ð PCR). Is there an antifoam which is compatible with most types of DNA
ð isolation reagents?
ð ---
Depending on the design of the mixer, it may have a foam arrestor attachment
(Waring blendors do), or you could make one yourself--a sheet of polypropylene
which covers the surface of the liquid and prevents air from entering the
liquid.
o----------------------------------------------------------------------o
| Dr. Peter Gegenheimer | Vox: 785-864-3939 FAX: 785-864-5321 |
| Department of | PGegen at UKans.nospam.edu |
| Molecular Biosciences | http://rnaworld.bio.ukans.edu/ |
| University of Kansas |"When you have excluded the impossible, |
| 2045 Haworth Hall | whatever remains, however improbable, |
| Lawrence KS 66045-2106 | must be the truth." S. Holmes |
o_____________________________|________________________________________o
More information about the Methods
mailing list