clonning

Liz Izquierdo liz.izquierdo at mailbox.uq.edu.au
Thu Sep 14 19:03:33 EST 2000


hi!!!
I appreciate any help....
I got a 3' fragment of a gene. Also, I got a small 5' of the same gene that
overlap eachother. I want to get full length fragment to clone it in a
vector
I tried to cut with an enzyme both fragments separately . When I run the
digestion on a gel the yield was very poor and also the samples were not
running straight. they wwere follow like a a wave What shall I do????
Dr. Liz Izquierdo
School of Land and Food
University of Queensland,
QLD. 4071. Autralia
Lab. Phone number:61-7-3365 1518
Office: 61-7-3365 1103
Fax: 61-7-3365 1177


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