Simple PCR isolation

Patrick G. Kaminker pgkaminker at lbl.gov
Fri Sep 15 14:39:26 EST 2000


This seems like a rather basic question, but it may save me some time.

After PCR with primers containing restriction sites added on, can you
immediately digest the PCR fragments prior to purifying by gel
extraction?  In other words can you avoid an extre gel purification or
PCR clean up step prior to restriction digest of the fragments?

Thank you.


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