DNA stick to agarose gel!

Michael Witty mw132 at mole.bio.cam.ac.uk
Mon Sep 18 07:59:39 EST 2000


I get that sometime in DNA extracts.  I put it down to "dentatured DNA"
stuck in the well or some fluorescent contaminant that copurified with the
DNA.  In any case I don't think it is something to worry about.  Is it
plant DNA?

 On Sat, 16 Sep 2000, Rajeswari Srinivasan wrote:

> Hi ,
> I extract DNA by CTAB method and purify by phenol chloroform
> extraction.After washing I dry the DNA to remove ethanol
> completely.Still,everytime, in agarose gel ,part of the DNA sticks to
> the well.Can anybody suggest me some remedy?
> Thanks in advance.
> Rajeswari
> 
> 
> 






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