DNA stick to agarose gel!
wasowicz at moskit.uwm.edu.pl
Tue Sep 19 06:08:37 EST 2000
Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:
>I get that sometime in DNA extracts. I put it down to "dentatured DNA"
>stuck in the well or some fluorescent contaminant that copurified with the
>DNA. In any case I don't think it is something to worry about. Is it
> On Sat, 16 Sep 2000, Rajeswari Srinivasan wrote:
>> Hi ,
>> I extract DNA by CTAB method and purify by phenol chloroform
>> extraction.After washing I dry the DNA to remove ethanol
>> completely.Still,everytime, in agarose gel ,part of the DNA sticks to
>> the well.Can anybody suggest me some remedy?
>> Thanks in advance.
It may happen that what you see in the well is high molecular mass DNA
which simply refuses to enter the gel because of much-too-long
Krzysztof Wasowicz, DVM, Ph.D.
Dept of Animal Anatomy
University of Agriculture and Technology
10-957 Olsztyn-Kortowo II
e-mail:wasowicz at moskit.art.olsztyn.pl
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