HiTrap Protein A question

Student mucineer at iname.com
Tue Sep 19 06:06:28 EST 2000


Can anyone please tell me how important it is to dialyse my samples in the
sodium phosphate buffer pH 7 recommended before loading them onto the HiTrap
Protein A column by Pharmacia? My samples are tissue culture supernatants?
Has anyone tried simply adjusting the pH of the supernatant to 7 and load it
onto the column? How does that affect the yield?

Thanks.








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