Ceramide ELISA

Jens Rainer Hansen jens.rainer at leo.dk
Tue Sep 19 07:49:48 EST 2000


> I have coated ELISA plates with Ceramide - 1000 - 100 - 10 and 0 ng/ml
(100
> mcl/well) using 2 coating buffres (ph 9.6 and 7.2).
>
> Hereafter we added a mouse anti ceramide antibody and a biotinylated
rabbit anti mouse antibody, TMB etc., with no result. All wells gave the
same as the "0" = bagground.
>
> Does anybody have experience with coating procedures using ceramide ?
>
   I know it is a lipide and not a protein.
>
   Thanks
> Jens R.








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