mRNA isolation kits

[Gauthier , Eric]

Before you buy any expensive kits, why don't you try the good old Chomczyski
and Sacchi procedure (Anal Biochem, 1987, 162: 156-159; Pflugers Archiv,
1997, 433[5]: 664-668)? These methods work very well with cultured cell
lines and whole tissue and the RNA can be used without any problem for
RT-PCR applications.

Eric R. Gauthier, Ph.D.
email: egauthier at neorcc.on.ca


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