HiTrap Protein A question

Robert Slany rslany at biologie.uni-erlangen.de
Wed Sep 20 03:55:54 EST 2000

Hi "Student",

    in my hands it works fine just to dilute the tissue culture supernatant
1:1 with PBS. Then shoot it directly through the column (I used protein G,
might be different for protA).

Should work.


 Dr. Robert Slany
 Friedrich Alexander Universitaet Erlangen
 Department of Genetics
 Staudtstrasse 5
 91058 Erlangen, Germany
 +49-9131-8528527(office), -8528526(fax), -8528523 (lab)
 +49-9131-45942(private), +49-179-5981660(mobile)
 rslany at biologie.uni-erlangen.de

"Student" <mucineer at iname.com> schrieb im Newsbeitrag
news:8q7hau$1mv$1 at oyez.ccc.nottingham.ac.uk...
> Can anyone please tell me how important it is to dialyse my samples in the
> sodium phosphate buffer pH 7 recommended before loading them onto the
> Protein A column by Pharmacia? My samples are tissue culture supernatants?
> Has anyone tried simply adjusting the pH of the supernatant to 7 and load
> onto the column? How does that affect the yield?
> Thanks.

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