HiTrap Protein A question

Robert Slany rslany at biologie.uni-erlangen.de
Wed Sep 20 03:55:54 EST 2000


Hi "Student",

    in my hands it works fine just to dilute the tissue culture supernatant
1:1 with PBS. Then shoot it directly through the column (I used protein G,
might be different for protA).

Should work.

Robert

--
****************************************************************
 Dr. Robert Slany
 Friedrich Alexander Universitaet Erlangen
 Department of Genetics
 Staudtstrasse 5
 91058 Erlangen, Germany
 +49-9131-8528527(office), -8528526(fax), -8528523 (lab)
 +49-9131-45942(private), +49-179-5981660(mobile)
 rslany at biologie.uni-erlangen.de
****************************************************************

"Student" <mucineer at iname.com> schrieb im Newsbeitrag
news:8q7hau$1mv$1 at oyez.ccc.nottingham.ac.uk...
> Can anyone please tell me how important it is to dialyse my samples in the
> sodium phosphate buffer pH 7 recommended before loading them onto the
HiTrap
> Protein A column by Pharmacia? My samples are tissue culture supernatants?
> Has anyone tried simply adjusting the pH of the supernatant to 7 and load
it
> onto the column? How does that affect the yield?
>
> Thanks.
>
>
>







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