TAE vs TBE

R. Jayakumar jakku at mrna.tn.nic.in
Wed Sep 20 06:00:22 EST 2000


I came across this information from some where.  But it seems, TAE buffer
being a weaker buffer, crack more easily than TBE. So when it is necessary
to run at high voltages, TBE is more often preferred, but resolution suffers
a bit.
   cheers
jayakumar

----- Original Message -----
From: Frauke Naumann <fnaumann at scan.genetik.uni-koeln.de>
To: <methods at hgmp.mrc.ac.uk>
Sent: Wednesday, September 20, 2000 1:43 PM
Subject: Re: TAE vs TBE


> Hi,
> TAE is supposed to lead to a better resolution of small fragments. But you
> have to use a peristaltic pump when running the gels overnight. The
> depurination step before southern blotting with 0,25 M HCl requires less
> time when TAE is used because it's a *weaker* buffering system.
> Hope that's a good enough explanation....
> Frauke
>
> John/Pam Bohmfalk schrieb in Nachricht ...
> >Please pardon the perhaps ignorant question.  In agarose gels, what are
> >the advantages of TAE or TBE?  I have been unable to find a good
> >explanation of why or when one buffer should be used over the other.
> >Thanks.
> >
> >John
> >jbohmfalk at hastings.edu
>
>
>
>


---






More information about the Methods mailing list