PCR and RFLP
Ma. Sheila M de Jesus
msmj at edsamail.com.ph
Fri Sep 22 20:53:16 EST 2000
I seek professional advice on this:
I am working on comparing patterns or polymorphism within hemophiliac patients using RFLP on PCR products. The pcr product from intron 13 generates a 96 bp product. Is the protocol logical?
Our lab is not capable of doing southern blotting but is capable of doing PCR . This is my research fellowship project and unfortunately, no one here can provide me advice. Quite a neophyte in the aspect of molecular biology but very familiar with PCR.
If you can share an RFLP protocol in the analysis of the porlymorphism is a big help.
Again, many thanks in advance.
mshmdeje at ustcc.ust.edu.ph
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