Sv: TAE vs TBE
jakku at mrna.tn.nic.in
Mon Sep 25 00:14:37 EST 2000
same here too... we used it shamelessly again and again :-))
----- Original Message -----
From: "David L Haviland" <David.L.Haviland at uth.tmc.edu>
To: <methods at hgmp.mrc.ac.uk>
Sent: Monday, September 25, 2000 7:46 AM
Subject: Re: Sv: TAE vs TBE
> Same here... The only time I don't re-use it is when there is a chance
> that what I'm doing might end up published somewhere, like a genomic
> Southern - then I change the TAE buffer. Otherwise, I'll run it 2-3
> Hope this helps,
> David L. Haviland, Ph.D., Asst. Prof. Immunology
> University of Texas - Houston, H.S.C.
> Institute of Molecular Medicine, R907
> 2121 W. Holcombe Blvd., Houston, TX 77030
> If everything seems to be going so well, you have obviously
> overlooked something.
> ----- Original Message -----
> From: "Jens Tornoe" <jens at tornoe.net>
> Date: Sunday, September 24, 2000 4:16 pm
> Subject: Sv: TAE vs TBE
> > Just a quick note: I have reused TAE several times without
> > problems. Didn't
> > know it wasn't possible.
> > Regards,
> > Jens
> > Dave Vadnais <davevadnais at home.com> skrev i en
> > nyhedsmeddelelse:RN7z5.10375$dZ2.3920563 at news3.rdc1.on.home.com...
> > > Another plus to using TBE is that you can reuse the buffer after
> > a run,
> > > while with TAE you cannot reuse it.
> > > I routinely reuse my TBE tank buffer 2 to 3 times before
> > chucking it out.
> > > Dave
> > >
> > > --
> > > Dave A. Vadnais
> > > Plant Biotechnology Division
> > > Department of Plant Agriculture
> > > University of Guelph
> > > Guelph ON
> > > N1H 8H1
> > > Work: (519) 824-4120 ext. 8185
> > > Fax: (519) 763-8933
> > > email: dvadnais at uoguelph.ca
> > >
> > >
> > > "John/Pam Bohmfalk" <bohmfalk at tcgcs.com> wrote in message
> > > news:bohmfalk-1909001752390001 at has-pm2-048.inetnebr.com...
> > > > Please pardon the perhaps ignorant question. In agarose gels,
> > what are
> > > > the advantages of TAE or TBE? I have been unable to find a good
> > > > explanation of why or when one buffer should be used over the
> > other.> > Thanks.
> > > >
> > > > John
> > > > jbohmfalk at hastings.edu
> > >
> > >
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