antibody affinity column

Oliver.Politz at SCHERING.DE Oliver.Politz at SCHERING.DE
Mon Sep 25 08:54:52 EST 2000



Dear Netters,
I would like to get some hints on how to prepare an affinity column using a
polyclonal antibody (affinity purified) and ProteinA (or G) beads. The main
question is what might give a better column: using a batch procedure to
bind antibody to proteinA and then crosslink with DMP or doing this
procedure by using a prepacked ProteinA column (i.e. HiTrap from apbiotech)
were I could better wash in between.
Any further suggestions are also welcome
Thanks in advance
Please send answers directly via email to Oliver.Politz at schering.de



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