TAE vs TBE

ChenHA hzhen at freeuk.com
Mon Sep 25 17:30:45 EST 2000



"Michael L. Sullivan" wrote:
> 
> I had heard that borate, present in TBE, can be inhibitory to some enzymes
> (eg. DNA ligase), so that if using DNA directly out of low melt agarose, it
> was better to use TAE.  My own experience was that ligations still worked
> OK in the presence of TBE, though.
> 

Interesting.  My own experience is that borate do inhibit ligation. 
Could you confirm that your ligation is done in TBE, and not after
purification which removed the TBE?  And have done a comparison
between TAE and TBE? I would be interested to know.


> Mike
> 
> >Please pardon the perhaps ignorant question.  In agarose gels, what are
> >the advantages of TAE or TBE?  I have been unable to find a good
> >explanation of why or when one buffer should be used over the other.
> >Thanks.
> >
> >John
> >jbohmfalk at hastings.edu
> 
> ---






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