Primer Design Survey
arun.apte at usa.net
Wed Sep 27 13:54:03 EST 2000
Primer Premier can design primers that will amplify across species by using
multiple sequence alignment with primer design.
"sam richards" <richards-4 at medctr.osu.EDU> wrote in message
news:39BE2FBC.7F5C90D at medctr.osu.edu...
> To All,
> I would like to survey all those who do PCR and sequencing primer
> design. I am gathering information for a workshop and would like to know
> what you think about primer design so I can put together a consensus of
> information. Please take a few minutes and respond to this survey.
> Thank you for your time.
> Sam Richards
> Ohio State University
> 1. What guidelines do you use in designing primers?
> 2. How do you prefer designing primers (i.e. do you use software,
> website, or do you eye-ball it)?
> 3. If you use a website; which one do you use?
> 4. If you use a software package; which one is it?
> 5. Are there other design programs that you have used?
> 6. Is there any additional information that you would like to give about
> primer design that is not covered in this survey?
More information about the Methods