Why do probes stick to RNA markers?

Jonathan Howarth jonathan.howarth at bbsrc.ac.uk
Thu Sep 28 09:11:51 EST 2000


Many, but not all, of our 32-P labelled probes stick to the marker lane
on our northern blots. This happens with Sigma and Gibco BRL RNA ladders
and the bands the probes hyb to don't correspond always to the marker
bands.

Does anyone else get this, and can anyone give an explanation as to
whats happening?

If you can help us out with this query e-mail me at
jonathan.howarth at bbsrc.ac.uk

Thanks for any ideas

Jonathan






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