radioisotopes vs. chemilumenesence

Arnoud van Vliet avvliet at
Fri Sep 29 15:37:00 EST 2000

Hi Paul

I have been working a lot on getting DIG-Northerns to work on bacterial RNA,
and I have it working now (most of the time). I can mail you a protocol
(with some tips) if you like.

hope this helps

"Paul Cullen" <Paul.Cullen at> wrote in message
news:39D1455C.42D8047E at
> Adam,
>      I've also been trying a non radioactive northern protocol - with no
> joy yet. I'm using the DIG system and also have been unable to find a
> comparison of the sensitivities.
> adam biales wrote:
> >
> > Does anyone know the comparitive sensitivities between radioisotopes and
> > chemilumenessence or fluorometrics.  I am trying to justify using a
> > nonradioactive northern protocol and I can't find information regarding
> > relative sensitivities.
> >
> > Thanks,
> > Adam Biales
> --
> Paul Cullen
> Bacterial Pathogenesis Research Group
> Monash University
> Australia

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