Ammonium sulfate precipitation problems?

Jack Dirri xiphoric at
Sun Apr 1 20:58:47 EST 2001

>From many reports in ammonium sulfate precipitaion, the precipitated
protein is usually not denatured and activity is recovered upon
redissolving the pellet. Can ammonium sulfate precipitaion fractionate
the denaturing protein ? I have the problems that the protein (total
protein from plant) is denatured by the extraction process and all
proteins are precipitated at 20% saturation. Is it because portein is in
denaturing form?


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