SacII ?

[Viczian Andras]

I think the trick is that the SstI is the isoschisomer of SacI not 
SacII.
Bye,
Andras

> Dear all,
> 
> 	We need to digest a plasmid with SacII, and the enzyme 
> doesn't work...we tried SstI (isoschizomer). Is there any trick ?
> 
> 	with many thanks....
> 
> JM
> 
>  
> Jean-Marc  REICHHART
> UPR 9022 CNRS     IBMC
> 15, rue Rene Descartes
> 67084 - Strasbourg Cedex
> 	FRANCE
> JM.Reichhart at ibmc.u-strasbg.fr
> phone   33 388 417 034
> fax     33 388 606 922
> 
> ---
> 


---