SacII ?

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Wed Apr 4 20:47:08 EST 2001


At 10:58 AM 4/4/01 +0100, JMR wrote:
>Dear all,
>
>	We need to digest a plasmid with SacII, and the enzyme 
>doesn't work...we tried SstI (isoschizomer). Is there any trick ?


Oh Oh ... SstI is the isoschizomer of SacI, and not of SacII. Therein lies
your answer ... er ... trick. ;-)
Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Molecular Biology,
Dept. of Chemistry & Biochemistry	
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu

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