sacII-typing mistake

Eric DUFOUR Eric.Dufour at cmm.ki.se
Thu Apr 5 04:23:43 EST 2001



JMR wrote:

> Dear all,
>
>         We need to digest a plasmid with SacII, and the enzyme
> doesn't work... Sorry that I made a typing mistake in my first mail.
> We tried the true isoschizomer SstII and it didn't work. We changed
> the batch, purchased from another company... Is there any trick ?

Can you try BtgI or DsaI (C/CPuPyGG) or MspAI (CMG/CKG) if there is no
other site for these enzymes on your plasmid and your insert ?
I've never used these enzymes so I cannot guaranty their efficiency...

Eric





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