Ghost bands on plasmid preps

Daniel MacArthur dmac125 at
Thu Apr 5 04:40:23 EST 2001


> Did you perform Phenol-chloroform extraction on your minipreps?
> Some years ago I have had the same problem and I solved the
> problem shorting the SDS-NaOH step (1-2 min.) and using Phe-chlorof 
> (I didn't routinely use Phe in my preps). 

No, I used an SDS/NaOH lysis solution, which may well have been the cause of
the problem. If it's not too much trouble, could you give me a little more
detail on the composition of the phenol-chloroform solution? I'd be quite
keen to try it out and see what happens.

Thanks in advance,



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