sergioal at bbm1.ucm.es
Thu Apr 5 04:08:29 EST 2001
Are you sure the site is there?
SacII is sensitive to citosine methylation, but the dcm site is not a part of
the SacII site..
> Dear all,
> We need to digest a plasmid with SacII, and the enzyme
> doesn't work... Sorry that I made a typing mistake in my first mail.
> We tried the true isoschizomer SstII and it didn't work. We changed
> the batch, purchased from another company... Is there any trick ?
> With many thanks....
> Jean-Marc REICHHART
> UPR 9022 CNRS IBMC
> 15, rue Rene Descartes
> 67084 - Strasbourg Cedex
> JM.Reichhart at ibmc.u-strasbg.fr
> phone 33 388 417 034
> fax 33 388 606 922
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