sacII-typing mistake

Sergio sergioal at bbm1.ucm.es
Thu Apr 5 04:08:29 EST 2001


Are you sure the site is there?
SacII is sensitive to citosine methylation, but the dcm site is not a part of
the SacII site..

Sergio

JMR wrote:

> Dear all,
>
>         We need to digest a plasmid with SacII, and the enzyme
> doesn't work... Sorry that I made a typing mistake in my first mail.
> We tried the true isoschizomer SstII and it didn't work. We changed
> the batch, purchased from another company... Is there any trick ?
>
>         With many thanks....
>
> JM
>
>
> Jean-Marc  REICHHART
> UPR 9022 CNRS     IBMC
> 15, rue Rene Descartes
> 67084 - Strasbourg Cedex
>         FRANCE
> JM.Reichhart at ibmc.u-strasbg.fr
> phone   33 388 417 034
> fax     33 388 606 922
>
> ---




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