alkaline pH of E. coli medium?
Dr. Duncan Clark
news at hgmp.mrc.ac.uk
Mon Apr 9 07:25:15 EST 2001
In article <3AD18BC8.BFFD3013 at bbm1.ucm.es>, the eminent Sergio at UCM
>When growing on LB, or any similar media (aminoacids as carbon source and no
>buffered), E. coli shifts the pH over 9, due to the aa metabolism (produces
But E.coli, depending upon genotype, also produces lots of acetate which
shifts the pH low? When feeding with glucose one normally controls the
pH with ammonium hydroxide i.e. it goes low.
In general the final pH is highly dependant upon both the growth media
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