alkaline pH of E. coli medium?

Dr. Duncan Clark news at
Mon Apr 9 07:25:15 EST 2001

In article <3AD18BC8.BFFD3013 at>, the eminent Sergio at UCM
>When growing on LB, or any similar media (aminoacids as carbon source and no
>buffered), E. coli shifts the pH over 9, due to the aa metabolism (produces

But E.coli, depending upon genotype, also produces lots of acetate which
shifts the pH low? When feeding with glucose one normally controls the
pH with ammonium hydroxide i.e. it goes low. 

In general the final pH is highly dependant upon both the growth media
and strain.

Homogeneous Fluorescent Reporting Systems for Real-Time Quantitative PCR: 

Optimisation, Probe Technology & Future Systems

4-5 September 2001
King Alfred's College, Winchester, UK

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