alkaline pH of E. coli medium?

Dr. Duncan Clark news at hgmp.mrc.ac.uk
Mon Apr 9 07:25:15 EST 2001


In article <3AD18BC8.BFFD3013 at bbm1.ucm.es>, the eminent Sergio at UCM
wrote
>When growing on LB, or any similar media (aminoacids as carbon source and no
>buffered), E. coli shifts the pH over 9, due to the aa metabolism (produces
>ammonium).

But E.coli, depending upon genotype, also produces lots of acetate which
shifts the pH low? When feeding with glucose one normally controls the
pH with ammonium hydroxide i.e. it goes low. 

In general the final pH is highly dependant upon both the growth media
and strain.

Duncan
-- 
Homogeneous Fluorescent Reporting Systems for Real-Time Quantitative PCR: 

Optimisation, Probe Technology & Future Systems

4-5 September 2001
King Alfred's College, Winchester, UK 

http://www.dera.gov.uk/html/news/events/real_time_quantitative_pcr.htm




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