Stability of sticky ends.

Dr. Hiranya S. Roychowdhury hroychow at
Mon Apr 9 21:19:32 EST 2001

Well, I think the stability depends on two things: 
1. Buffering
2. cleanliness.

If those two standards are met, restrtiction digested DNA may be stored
almost indefinitely. I have vector preps from 1993 that I am still using
with no noticeable difference in ligatability  (is that a legit word?). 

At 05:28 PM 4/9/01 GMT, CHMC wrote:
>There is someone in my lab who likes to restriction digest a lot of DNA and
>save it in the freezer.  I believe that the sticky ends are not completely
>stable, and tend to go away over time.  However, I can't remember where I
>learned that so I don't know if it is true or just my superstition.
>Does anyone have any information on the stability of sticky ends?
Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Molecular Biology,
Dept. of Chemistry & Biochemistry	
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at


More information about the Methods mailing list